smallRNA-seq of Nomia melanderi: adult female brain - SRA

SRX6701638: smallRNA-seq of Nomia melanderi: adult female brain
1 ILLUMINA (Illumina HiSeq 2500) run: 22.4M spots, 542.2M bases, 242.1Mb downloads

Design: Head capsules from the B. impatiens, M. genalis, and N. melanderi samples were dissected after incubation in RNALater ICE (Ambion) to remove the entire brain. We used the mirVana miRNA Isolation kit with phenol (Ambion) to isolate total RNA from individual brains. Total RNA was sent to the University of Illinois Roy J. Carver Biotechnology Center for library preparation and sequencing. Libraries were prepared with the Illumina TruSeq Small RNA Sample Preparation kit. Libraries were pooled, quantitated by qPCR, and sequenced on one lane for 51 cycles on a HiSeq 2500, using TruSeq SBS sequencing kit version 2.

Submitted by: Utah State University

Study: Brain microRNA expression in six bee species

PRJNA559906 • SRP218141 • All experiments • All runs

show Abstracthide Abstract

In this study, we sequenced small RNA from whole brains of six bee species to study microRNA evolution associated with eusociality.

Sample: Nomia melanderi female 8

SAMN12562769 • SRS5256587 • All experiments • All runs

Organism: Nomia melanderi

Library:

Name: Nomia_melanderi_8_TTAGGCAT_L005_R1_001

Instrument: Illumina HiSeq 2500

Strategy: miRNA-Seq